Induction of the CD24 surface antigen in primary undifferentiated human adipose progenitor cells by the hedgehog signaling pathway
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Muoio, Francesco
ORCID
Foundation for Cardiological Research and Education (FCRE), Cardiocentro Ticino, Taverne, Switzerland
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Panella, Stefano
ORCID
Foundation for Cardiological Research and Education (FCRE), Cardiocentro Ticino, Taverne, Switzerland
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Harder, Yves
ORCID
Department of Plastic, Reconstructive, and Aesthetic Surgery, EOC, Lugano, Switzerland - Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
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Tallone, Tiziano
ORCID
Foundation for Cardiological Research and Education (FCRE), Cardiocentro Ticino, Taverne, Switzerland
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Published in:
- Biologics. - 2021, vol. 1, no. 2, p. 129-153
English
In the murine model system of adipogenesis, the CD24 cell surface protein represents a valuable marker to label undifferentiated adipose progenitor cells. Indeed, when injected into the residual fat pads of lipodystrophic mice, these CD24 positive cells reconstitute a normal white adipose tissue (WAT) depot. Unluckily, similar studies in humans are rare and incomplete. This is because it is impossible to obtain large numbers of primary CD24 positive human adipose stem cells (hASCs). This study shows that primary hASCs start to express the glycosylphosphatidylinositol (GPI)-anchored CD24 protein when cultured with a chemically defined medium supplemented with molecules that activate the Hedgehog (Hh) signaling pathway. Therefore, this in vitro system may help understand the biology and role in adipogenesis of the CD24-positive hASCs. The induced cells’ phenotype was studied by flow cytometry, Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) techniques, and their secretion profile. The results show that CD24 positive cells are early undifferentiated progenitors expressing molecules related to the angiogenic pathway.
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Medicine
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CC BY
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Open access status
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gold
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Persistent URL
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https://n2t.net/ark:/12658/srd1322960
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