Journal article

Rationally modified estrogen receptor protein as a bio-recognition element for the detection of EDC pollutants : strategies and opportunities

  • Pedotti, Mattia Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
  • Ferrero, Valentina Elisabetta Viviana European Commission—Directorate General—Joint Research Centre, Institute for Environment and Sustainability, Ispra, Varese, Italy
  • Lettieri, Teresa European Commission—Directorate General—Joint Research Centre, Institute for Environment and Sustainability, Ispra, Varese, Italy
  • Colpo, Pascal European Commission—Directorate General—Joint Research Centre, Institute for Health and Consumer Protection, Ispra, Varese, Italy
  • Follonier, Stephane CSEM Landquart, Bahnhofstrasse 1, Landquart, Switzerland
  • Calzolai, Luigi European Commission—Directorate General—Joint Research Centre, Institute for Health and Consumer Protection, Ispra, Varese, Italy
  • Varani, Luca Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
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    27.02.2015
Published in:
  • International journal of environmental research and public health. - 2015, vol. 12, no. 3, p. 2612-2621
English The estrogen receptor protein (ER) can bind a vast number of organic pollutants widely spread in the environment and collectively known as Endocrine Disrupting Chemicals, EDCs. Its broad selectivity makes it an ideal bio-recognition element for the detection of EDCs. Here we describe the strategy and rationale for the design of ER based biosensors and assays that generate a signal in the presence of EDCs. The opportunity to use either natural or rationally modified ER molecules is discussed. The latter approach was successfully applied in the EU-FP7 project RADAR, with the aim to develop a novel biosensor for the detection of organic pollutants both in the environment and in commercial water products.
Language
  • English
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Medicine
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https://susi.usi.ch/usi/documents/319014
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