The motor activity of DNA2 functions as an ssDNA translocase to promote DNA end resection
- Levikova, Maryna Institute of Molecular Cancer Research, University of Zurich, 8057 Zurich, Switzerland
- Pinto, Cosimo Institute of Molecular Cancer Research, University of Zurich, 8057 Zurich, Switzerland
- Cejka, Petr Institute of Molecular Cancer Research, University of Zurich, 8057 Zurich, Switzerland - Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
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23.03.2017
Published in:
- Genes and Development. - Cold Spring Harbor Laboratory Press. - 2017, vol. 31, no. 5, p. 493-502
English
DNA2 nuclease–helicase functions in DNA replication and recombination. This requires the nuclease of DNA2, while, in contrast, the role of the helicase activity has been unclear. We now show that the motor activity of both recombinant yeast and human DNA2 promotes efficient degradation of long stretches of ssDNA, particularly in the presence of the replication protein A. This degradation is further stimulated by a direct interaction with a cognate RecQ family helicase, which functions with DNA2 in DNA end resection to initiate homologous recombination. Consequently, helicase- deficient yeast dna2 K1080E cells display reduced resection speed of HO-induced DNA double-strand breaks. These results support a model of DNA2 and the RecQ family helicase partner forming a bidirectional motor machine, where the RecQ family helicase is the lead helicase, and the motor of DNA2 functions as a ssDNA translocase to promote degradation of 5'-terminated DNA.
- Language
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- English
- Classification
- Biological sciences
- License
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CC BY-NC
- Open access status
- hybrid
- Identifiers
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- RERO DOC 324121
- ARK ark:/12658/srd1318829
- Persistent URL
- https://n2t.net/ark:/12658/srd1318829
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