Journal article
Using triplet-triplet energy transfer to measure conformational dynamics in polypeptide chains.
- Fierz B Department of Biophysical Chemistry, Biozentrum, University of Basel, Basel, Switzerland.
- Joder K
- Krieger F
- Kiefhaber T
- 2006-09-08
Published in:
- Methods in molecular biology (Clifton, N.J.). - 2007
Amino Acids
Kinetics
Models, Molecular
Peptides
Photochemistry
Protein Conformation
Protein Folding
Xanthones
English
Intrachain diffusion processes play an important role in protein folding and function. In this chapter we discuss the application of triplet-triplet energy transfer to directly measure rate constants for intrachain contact formation in polypeptide chains. The photochemistry of triplet-triplet energy transfer is described, experimental prerequisites of the method are discussed, and a detailed description of the experimental protocols and data analysis is given.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1385/1-59745-189-4:169
- PMID 16957323
- Persistent URL
- https://susi.usi.ch/global/documents/143069
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