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The quenching of electrochemiluminescence upon oligonucleotide hybridization.
Journal article

The quenching of electrochemiluminescence upon oligonucleotide hybridization.

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  • 2004-09-28
Published in:
  • Luminescence : the journal of biological and chemical luminescence. - 2004
Carbocyanines
Electrochemistry
Genome
Luminescent Measurements
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis
Oligonucleotides
Organometallic Compounds
Sensitivity and Specificity
English Many genomic assays rely on a distance-dependent interaction between luminescent labels, such as luminescence quenching or resonance energy transfer. We studied the interaction between electrochemically excited Ru(bpy)(3) (2+) and Cy5 in a hybridization assay on a chip. The 3' end of an oligonucleotide was labelled with Ru(bpy)(3) (2+) and the 5' end of a complementary strand with Cy5. Upon the hybridization, the electrochemiluminescence (ECL) of Ru(bpy)(3) (2+) was efficiently quenched by Cy5 with a sensitivity down to 30 nmol/L of the Cy5-labelled complementary strand. The quenching efficiency is calculated to be 78%. A similar phenomenon was observed in a comparative study using laser-excitation of Ru(bpy)(3) (2+). The hybridization with the non-labelled complementary or labelled non-complementary strand did not change the intensity of the ECL signal. Resonance energy transfer, electron transfer and static quenching mechanisms are discussed. Our results suggest that static quenching and/or electron transfer are the most likely quenching mechanisms.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://susi.usi.ch/global/documents/126543
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