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Carbone_2023_MDPI_Cancers_Potential of miRNAs in Plasma
Journal article

Potential of miRNAs in plasma extracellular vesicle for the stratification of prostate cancer in a South African population

  • Temilola, Dada Oluwaseyi International Centre for Genetic Engineering and Biotechnology (ICGEB), Cape Town, South Africa - Integrative Biomedical Sciences Division, Faculty of Health Sciences, University of Cape Town, South Africa
  • Wium, Martha International Centre for Genetic Engineering and Biotechnology (ICGEB), Cape Town, South Africa
  • Paccez, Juliano International Centre for Genetic Engineering and Biotechnology (ICGEB), Cape Town, South Africa
  • Salukazana, Azola Samkele Division of Urology, University of Cape Town, Groote Schuur Hospital, South Africa
  • Otu, Hasan H. Department of Electrical and Computer Engineering, University of Nebraska-Lincoln, USA
  • Carbone, Giuseppina M. ORCID Institute of Oncology Research (IOR), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
  • Kaestner, Lisa Division of Urology, University of Cape Town, Groote Schuur Hospital, South Africa
  • Cacciatore, Stefano International Centre for Genetic Engineering and Biotechnology (ICGEB), Cape Town, South Africa
  • Zerbini, Luiz Fernando International Centre for Genetic Engineering and Biotechnology (ICGEB), Cape Town, South Africa
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  • 2023
Published in:
  • Cancers. - 2023, vol. 15, no. 15, p. 3968
Prostate cancer
Extracellular vesicles
Exosomes
miRNA
miR-194-5p
miR-16-5p
South Africa
English Prostate cancer (PCa) is the most common cause of cancer death among African men. The analysis of microRNAs (miRNAs) in plasma extracellular vesicles (EVs) can be utilized as a non-invasive tool for the diagnosis of PCa. In this study, we used small RNA sequencing to profile miRNAs cargo in plasma EVs from South African PCa patients. We evaluated the differential expression of miRNAs between low and high Gleason scores in the plasma EVs of South African patients and in the prostatic tissue from data available in the Cancer Genome Atlas (TCGA) Data Portal. We identified 7 miRNAs differently expressed in both EVs and prostatic tissues. We evaluated their expression using qPCR in a larger cohort of 10 patients with benign prostatic hyperplasia (BPH) and 24 patients with PCa. Here, we reported that the ratio between two of these miRNAs (i.e., miR-194-5p/miR-16-5p) showed a higher concentration in PCa compared to BPH and in metastatic PCa compared to localized PCa. We explored for the first time the profiling of miRNAs cargo in plasma EVs as a tool for the identification of putative markers in the South African population. Our finding indicated the ratio miR-194-5p/miR-16-5p as a non-invasive marker for the evaluation of PCa aggressiveness in this population.
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Language
  • English
Classification
Medicine
License
CC BY
Open access status
gold
Identifiers
Persistent URL
https://n2t.net/ark:/12658/srd1331852
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