Memory T cells in latent mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset
- Lindestam Arlehamn, Cecilia S. La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- Gerasimova, Anna La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- Mele, Federico Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
- Henderson, Ryan La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- Swann, Justine La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- Greenbaum, Jason A. La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- Kim, Yohan La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- Sidney, John La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- James, Eddie A. Benaroya Research Institute, Seattle, Washington, United States of America
- Taplitz, Randy Antiviral Research Centre, University of California, San Diego, San Diego, California, United States of America
- McKinney, Denise M. La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- Kwok, William W. Benaroya Research Institute, Seattle, Washington, United States of America
- Grey, Howard La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- Sallusto, Federica Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
- Peters, Bjoern La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
- Sette, Alessandro La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
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24.01.2013
Published in:
- Plos pathogens. - 2013, vol. 9, no. 1, p. e1003130
English
An understanding of the immunological footprint of Mycobacterium tuberculosis (MTB) CD4 T cell recognition is still incomplete. Here we report that human Th1 cells specific for MTB are largely contained in a CXCR3+CCR6+ memory subset and highly focused on three broadly immunodominant antigenic islands, all related to bacterial secretion systems. Our results refute the notion that secreted antigens act as a decoy, since both secreted proteins and proteins comprising the secretion system itself are targeted by a fully functional T cell response. In addition, several novel T cell antigens were identified which can be of potential diagnostic use, or as vaccine antigens. These results underline the power of a truly unbiased, genome-wide, analysis of CD4 MTB recognition based on the combined use of epitope predictions, high throughput ELISPOT, and T cell libraries using PBMCs from individuals latently infected with MTB.
- Language
-
- English
- Classification
- Medicine
- License
- CC BY
- Open access status
- gold
- Identifiers
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- RERO DOC 326694
- DOI 10.1371/journal.ppat.1003130
- ARK ark:/12658/srd1319175
- Persistent URL
- https://n2t.net/ark:/12658/srd1319175
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