Journal article

Memory T cells in latent mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset

  • Lindestam Arlehamn, Cecilia S. La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • Gerasimova, Anna La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • Mele, Federico Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
  • Henderson, Ryan La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • Swann, Justine La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • Greenbaum, Jason A. La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • Kim, Yohan La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • Sidney, John La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • James, Eddie A. Benaroya Research Institute, Seattle, Washington, United States of America
  • Taplitz, Randy Antiviral Research Centre, University of California, San Diego, San Diego, California, United States of America
  • McKinney, Denise M. La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • Kwok, William W. Benaroya Research Institute, Seattle, Washington, United States of America
  • Grey, Howard La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • Sallusto, Federica Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
  • Peters, Bjoern La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
  • Sette, Alessandro La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America
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    24.01.2013
Published in:
  • Plos pathogens. - 2013, vol. 9, no. 1, p. e1003130
English An understanding of the immunological footprint of Mycobacterium tuberculosis (MTB) CD4 T cell recognition is still incomplete. Here we report that human Th1 cells specific for MTB are largely contained in a CXCR3+CCR6+ memory subset and highly focused on three broadly immunodominant antigenic islands, all related to bacterial secretion systems. Our results refute the notion that secreted antigens act as a decoy, since both secreted proteins and proteins comprising the secretion system itself are targeted by a fully functional T cell response. In addition, several novel T cell antigens were identified which can be of potential diagnostic use, or as vaccine antigens. These results underline the power of a truly unbiased, genome-wide, analysis of CD4 MTB recognition based on the combined use of epitope predictions, high throughput ELISPOT, and T cell libraries using PBMCs from individuals latently infected with MTB.
Language
  • English
Classification
Medicine
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https://susi.usi.ch/usi/documents/319175
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