Journal article

Influenza hemagglutinin membrane anchor

  • Benton, Donald J. Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT London, United Kingdom
  • Nans, Andrea Structural Biology of Cells and Viruses Laboratory, Francis Crick Institute, NW1 1AT London, United Kingdom - Structural Biology Science Technology Platform, Francis Crick Institute, NW1 1AT London, United Kingdom
  • Calder, Lesley J. Structural Biology of Cells and Viruses Laboratory, Francis Crick Institute, NW1 1AT London, United Kingdom
  • Turner, Jack Structural Biology of Cells and Viruses Laboratory, Francis Crick Institute, NW1 1AT London, United Kingdom
  • Neu, Ursula Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT London, United Kingdom
  • Lin, Yi Pu Worldwide Influenza Centre, Francis Crick Institute, NW1 1AT London, United Kingdom
  • Ketelaars, Esther Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
  • Kallewaard, Nicole L. MedImmune LLC, Gaithersburg, MD 20878
  • Corti, Davide Humabs BioMed SA, 6500 Bellinzona, Switzerland
  • Lanzavecchia, Antonio Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
  • Gamblin, Steven J. Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT London, United Kingdom
  • Rosenthal, Peter B. Structural Biology of Cells and Viruses Laboratory, Francis Crick Institute, NW1 1AT London, United Kingdom
  • Skehel, John J. Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT London, United Kingdom
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    17.09.2018
Published in:
  • Proceedings of the national academy of sciences of the United States of America. - 2018, vol. 115, no. 40, p. 10112-10117
English Viruses with membranes fuse them with cellular membranes, to transfer their genomes into cells at the beginning of infection. For Influenza virus, the membrane glycoprotein involved in fusion is the hemagglutinin (HA), the 3D structure of which is known from X-ray crystallographic studies. The soluble ectodomain fragments used in these studies lacked the “membrane anchor” portion of the molecule. Since this region has a role in membrane fusion, we have determined its structure by analyzing the intact, full-length molecule in a detergent micelle, using cryo-EM. We have also compared the structures of full-length HA−detergent micelles with full-length HA−Fab complex detergent micelles, to describe an infectivity-neutralizing monoclonal Fab that binds near the ectodomain membrane anchor junction. We determine a high- resolution HA structure which compares favorably in detail with the structure of the ectodomain seen by X-ray crystallography; we detect, clearly, all five carbohydrate side chains of HA; and we find that the ectodomain is joined to the membrane anchor by flexible, eight-residue-long, linkers. The linkers extend into the detergent micelle to join a central triple-helical structure that is a major component of the membrane anchor.
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  • English
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Medicine
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https://susi.usi.ch/usi/documents/319000
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