CCR6 is expressed on an IL-10-producing, autoreactive memory T cell population with context-dependent regulatory function
-
Rivino, Laura
Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland - Charité Research Center for ImmunoSciences and German Rheumatism Research Center, Campus Charité Mitte, 10117 Berlin, Germany
-
Gruarin, Paola
Istituto Nazionale di Genetica Molecolare, 20122 Milan, Italy
-
Häringer, Barbara
Charité Research Center for ImmunoSciences and German Rheumatism Research Center, Campus Charité Mitte, 10117 Berlin, Germany
-
Steinfelder, Svenja
Charité Research Center for ImmunoSciences and German Rheumatism Research Center, Campus Charité Mitte, 10117 Berlin, Germany
-
Lozza, Laura
Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland - Charité Research Center for ImmunoSciences and German Rheumatism Research Center, Campus Charité Mitte, 10117 Berlin, Germany
-
Steckel, Bodo
Charité Research Center for ImmunoSciences and German Rheumatism Research Center, Campus Charité Mitte, 10117 Berlin, Germany
-
Weick, Anja
Charité Research Center for ImmunoSciences and German Rheumatism Research Center, Campus Charité Mitte, 10117 Berlin, Germany
-
Sugliano, Elisa
Istituto Nazionale di Genetica Molecolare, 20122 Milan, Italy
-
Jarrossay, David
Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
-
Kühl, Anja A.
Charité Research Center for ImmunoSciences/Institute of Pathology, Campus Benjamin Franklin, 12200 Berlin, Germany
-
Loddenkemper, Christoph
Charité Research Center for ImmunoSciences/Institute of Pathology, Campus Benjamin Franklin, 12200 Berlin, Germany - Institute of Pathology, Technische Universität München, 81625 Munich, Germany
-
Abrignani, Sergio
Istituto Nazionale di Genetica Molecolare, 20122 Milan, Italy
-
Sallusto, Federica
Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
-
Lanzavecchia, Antonio
Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
-
Geginat, Jens
Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland - Charité Research Center for ImmunoSciences and German Rheumatism Research Center, Campus Charité Mitte, 10117 Berlin, Germany - Istituto Nazionale di Genetica Molecolare, 20122 Milan, Italy
Show more…
Published in:
- Journal of experimental medicine. - 2010, vol. 207, no. 3, p. 565-577
English
Interleukin (IL)-10 produced by regulatory T cell subsets is important for the prevention of autoimmunity and immunopathology, but little is known about the phenotype and function of IL-10–producing memory T cells. Human CD4+CCR6+ memory T cells contained comparable numbers of IL-17– and IL-10–producing cells, and CCR6 was induced under both Th17-promoting conditions and upon tolerogenic T cell priming with transforming growth factor (TGF)–. In normal human spleens, the majority of CCR6+ memory T cells were in the close vicinity of CCR6+ myeloid dendritic cells (mDCs), and strikingly, some of them were secreting IL-10 in situ. Furthermore, CCR6+ memory T cells produced suppressive IL-10 but not IL-2 upon stimulation with autologous immature mDCs ex vivo, and secreted IL-10 efficiently in response to suboptimal T cell receptor (TCR) stimulation with anti-CD3 antibodies. However, optimal TCR stimulation of CCR6+ T cells induced expression of IL-2, interferon-, CCL20, and CD40L, and autoreactive CCR6+ T cell lines responded to various recall antigens. Notably, we isolated autoreactive CCR6+ T cell clones with context-dependent behavior that produced IL-10 with autologous mDCs alone, but that secreted IL-2 and proliferated upon stimulation with tetanus toxoid. We propose the novel concept that a population of memory T cells, which is fully equipped to participate in secondary immune responses upon recognition of a relevant recall antigen, contributes to the maintenance of tolerance under steady-state conditions.
-
Language
-
-
Classification
-
Medicine
-
License
-
CC BY-NC-SA
-
Open access status
-
hybrid
-
Identifiers
-
-
RERO DOC
324215
-
ARK
ark:/12658/srd1318954
-
Persistent URL
-
https://n2t.net/ark:/12658/srd1318954
Statistics
Document views: 46
File downloads: