Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone
- Pickett, Julie E. Division of Nuclear Medicine and Molecular Imaging, Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Gaithersburg, MD, USA
- Thompson, John M. Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
- Sadowska, Agnieszka Department of Infectious Disease, MedImmune LLC, Gaithersburg, MD, USA
- Tkaczyk, Christine Department of Infectious Disease, MedImmune LLC, Gaithersburg, MD, USA
- Sellman, Bret R. Department of Infectious Disease, MedImmune LLC, Gaithersburg, MD, USA
- Minola, Andrea Humabs BioMed SA, Bellinzona, Switzerland
- Corti, Davide Humabs BioMed SA, Bellinzona, Switzerland
- Lanzavecchia, Antonio Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
- Miller, Lloyd S. Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA - Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA - Division of Infectious Disease, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
- Thorek, Daniel LJ Division of Nuclear Medicine and Molecular Imaging, Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Gaithersburg, MD, USA - Cancer Molecular and Functional Imaging Program, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
-
25.04.2018
Published in:
- Bone research. - 2018, vol. 6, p. 13
English
Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to evaluate a novel human monoclonal antibody (mAb) probe directed against the Gram-positive bacterial surface molecule lipoteichoic acid (LTA). Specificity and affinity were assessed in vitro. We then radiolabeled the anti-LTA mAb and evaluated its effectiveness as a diagnostic imaging tool for detecting infection via immunoPET imaging in an in vivo mouse model of prosthetic joint infection (PJI). In vitro and ex vivo binding of the anti-LTA mAb to pathogenic bacteria was measured with Octet, ELISA, and flow cytometry. The in vivo PJI mouse model was assessed using traditional imaging modalities, including positron emission tomography (PET) with [18F]FDG and [18F]NaF as well as X-ray computed tomography (CT), before being evaluated with the zirconium-89-labeled antibody specific for LTA ([89Zr]SAC55). The anti-LTA mAb exhibited specific binding in vitro to LTA-expressing bacteria. Results from imaging showed that our model could reliably simulate infection at the surgical site by bioluminescent imaging, conventional PET tracer imaging, and bone morphological changes by CT. One day following injection of both the radiolabeled anti-LTA and isotype control antibodies, the anti-LTA antibody demonstrated significantly greater (P < 0.05) uptake at S. aureus-infected prosthesis sites over either the same antibody at sterile prosthesis sites or of control non-specific antibody at infected prosthesis sites. Taken together, the radiolabeled anti-LTA mAb, [89Zr]SAC55, may serve as a valuable diagnostic molecular imaging probe to help distinguish between sterile inflammation and infection in the setting of PJI. Future studies are needed to determine whether these findings will translate to human PJI.
- Language
-
- English
- Classification
- Medicine
- License
- License undefined
- Identifiers
-
- RERO DOC 326705
- DOI 10.1038/s41413-018-0014-y
- ARK ark:/12658/srd1318927
- Persistent URL
- https://n2t.net/ark:/12658/srd1318927
Statistics
Document views: 78
File downloads:
- Pickett_BR_2018.pdf: 106