De Felice, Alessandra Aureli, Simone Limongelli, Vittorio 2021 G protein-coupled receptors (GPCRs) are the largest human membrane receptor family regulating a wide range of cell signaling. For this reason, GPCRs are highly desirable drug targets, with approximately 40% of prescribed medicines targeting a member of this receptor family. The structural homology of GPCRs and the broad spectrum of applications of GPCR-acting drugs suggest an investigation of the cross-activity of a drug toward different GPCR receptors with the aim of rationalizing drug side effects, designing more selective and less toxic compounds, and possibly proposing off-label therapeutic applications. Herein, we present an original in silico approach named “Computational Profiling for GPCRs” (CPG), which is able to represent, in a one-dimensional (1D) string, the physico-chemical properties of a ligand–GPCR binding interaction and, through a tailored alignment algorithm, repurpose the ligand for a different GPCR. We show three case studies where docking calculations and pharmacological data confirm the drug repurposing findings obtained through CPG on 5-hydroxytryptamine receptor 2B, beta-2 adrenergic receptor, and M2 muscarinic acetylcholine receptor. The CPG code is released as a user-friendly graphical user interface with numerous options that make CPG a powerful tool to assist the drug design of GPCR ligands. application/pdf https://n2t.net/ark:/12658/srd1328323 https://susi.usi.ch/global/documents/328323 https://susi.usi.ch/documents/328323/files/De Felice_2021_fmolb.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.3389/fmolb.2021.673053 info:eu-repo/semantics/altIdentifier/ark/12658/srd1328323 info:eu-repo/semantics/openAccess CC BY Frontiers in molecular biosciences . - 2021, vol. 8, p. 673053 info:eu-repo/classification/udc/615 Drug repurposing on G protein-coupled receptors using a computational profiling approach http://purl.org/coar/resource_type/c_6501