Lanzavecchia, Antonio Frühwirth, Alexander Perez, Laurent Corti, Davide 2016-06-22 In the last decade, progress in the analysis of the human immune response and in the isolation of human monoclonal antibodies have provided an innovative approach to the identification of protective antigens which are the basis for the design of vaccines capable of eliciting effective B-cell immunity. In this review we illustrate, with relevant examples, the power of this approach that can rapidly lead to the identification of protective antigens in complex pathogens, such as human cytomegalovirus and Plasmodium falciparum, and of conserved sites in highly variable antigens, such as influenza hemagglutinin and HIV-1 Env. We will also discuss how the genealogical analysis of antigen-stimulated B cell clones provides the basis to delineate the best suitable prime-boost vaccination strategy for the induction of broadly neutralizing antibodies. application/pdf https://n2t.net/ark:/12658/srd1319368 https://susi.usi.ch/global/documents/319368 https://susi.usi.ch/documents/319368/files/Lanzavecchia_2020_curropinimmunol.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.coi.2016.06.001 info:eu-repo/semantics/altIdentifier/ark/12658/srd1319368 info:eu-repo/semantics/openAccess CC BY-NC-ND Current opinion in immunology. - 2016, vol. 41, no. August, p. 62-67 info:eu-repo/classification/udc/61 Antibody-guided vaccine design : identification of protective epitopes http://purl.org/coar/resource_type/c_6501