Lindestam Arlehamn, Cecilia S. Gerasimova, Anna Mele, Federico Henderson, Ryan Swann, Justine Greenbaum, Jason A. Kim, Yohan Sidney, John James, Eddie A. Taplitz, Randy McKinney, Denise M. Kwok, William W. Grey, Howard Sallusto, Federica Peters, Bjoern Sette, Alessandro 2013-01-24 An understanding of the immunological footprint of Mycobacterium tuberculosis (MTB) CD4 T cell recognition is still incomplete. Here we report that human Th1 cells specific for MTB are largely contained in a CXCR3+CCR6+ memory subset and highly focused on three broadly immunodominant antigenic islands, all related to bacterial secretion systems. Our results refute the notion that secreted antigens act as a decoy, since both secreted proteins and proteins comprising the secretion system itself are targeted by a fully functional T cell response. In addition, several novel T cell antigens were identified which can be of potential diagnostic use, or as vaccine antigens. These results underline the power of a truly unbiased, genome-wide, analysis of CD4 MTB recognition based on the combined use of epitope predictions, high throughput ELISPOT, and T cell libraries using PBMCs from individuals latently infected with MTB. application/pdf https://susi.usi.ch/global/documents/319175 https://n2t.net/ark:/12658/srd1319175 https://susi.usi.ch/documents/319175/files/Lindestam_PP_2013.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.ppat.1003130 info:eu-repo/semantics/altIdentifier/ark/12658/srd1319175 info:eu-repo/semantics/openAccess CC BY Plos pathogens. - 2013, vol. 9, no. 1, p. e1003130 info:eu-repo/classification/udc/61 Memory T cells in latent mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset http://purl.org/coar/resource_type/c_6501