Campana, Raffaela Moritz, Katharina Neubauer, Angela Huber, Hans Henning, Rainer Brodie, Tess M. Kaider, Alexandra Sallusto, Federica Wöhrl, Stefan Valenta, Rudolf 2017-09-14 The effects of epicutaneous allergen administration on systemic immune responses in allergic and non-allergic individuals has not been investigated with defined allergen molecules. We studied the effects of epicutaneous administration of rBet v 1 and rBet v 1 fragments on systemic immune responses in allergic and non-allergic subjects. We conducted a clinical trial in which rBet v 1 and two hypoallergenic rBet v 1 fragments were applied epicutaneously by atopy patch testing (APT) to 15 birch pollen (bp) allergic patients suffering from atopic dermatitis, 5 bp-allergic patients suffering from rhinoconjunctivitis only, 5 patients with respiratory allergy without bp allergy and 5 non-allergic individuals. Epicutaneous administration of rBet v 1 and rBet v 1 fragments led to strong and significant increases of allergen-specific T cell proliferation (CLA+ and CCR4+T cell responses) only in bp-allergic patients with a positive APT reaction. There were no relevant changes of Bet v 1-specific IgE and IgG responses. No changes were noted in allergic subjects without bp allergy and in non-allergic subjects. Epicutaneous allergen application boosts specific T cell but not antibody responses mainly in allergic, APT-positive patients suggesting IgE-facilitated allergen presentation as mechanism for its effects on systemic allergen-specific immune responses. application/pdf https://susi.usi.ch/global/documents/319148 https://localhost:5000/ark:/12658/srd1319148 https://susi.usi.ch/documents/319148/files/Campana_SR_2017.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-10278-1 info:eu-repo/semantics/altIdentifier/ark/12658/srd1319148 info:eu-repo/semantics/openAccess CC BY Scientific reports. - 2017, vol. 7, p. 11657 info:eu-repo/classification/udc/61 Epicutaneous allergen application preferentially boosts specific T cell responses in sensitized patients http://purl.org/coar/resource_type/c_6501