Kim, Jun Hyun Grosbart, Malgorzata Anand, Roopesh Wyman, Claire Cejka, Petr Petrini, John H.J. 2017-01-10 The Mre11 complex (Mre11, Rad50, and Nbs1) is integral to both DNA repair and ataxia telangiectasia mutated (ATM)-dependent DNA damage signaling. All three Mre11 complex components are essential for viability at the cellular and organismal levels. To delineate essential and non-essential Mre11 complex functions that are mediated by Nbs1, we used TALEN-based genome editing to derive Nbs1 mutant mice (Nbs1mid mice), which harbor mutations in the Mre11 interaction domain of Nbs1. Nbs1mid alleles that abolished interaction were incompatible with viability. Conversely, a 108-amino-acid Nbs1 fragment comprising the Mre11 interface was sufficient to rescue viability and ATM activation in cultured cells and support differentiation of hematopoietic cells in vivo. These data indicate that the essential role of Nbs1 is via its interaction with Mre11 and that most of the Nbs1 protein is dispensable for Mre11 complex functions and suggest that Mre11 and Rad50 directly activate ATM. application/pdf https://n2t.net/ark:/12658/srd1319018 https://susi.usi.ch/global/documents/319018 https://susi.usi.ch/documents/319018/files/Kim_CR_2017.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2016.12.035 info:eu-repo/semantics/altIdentifier/ark/12658/srd1319018 info:eu-repo/semantics/openAccess CC BY Cell reports. - 2017, vol. 18, no. 2, p. 496-507 Nbs1mid mutants Mre11-Nbs1 interface ATM activation Mre11 complex assembly info:eu-repo/classification/udc/61 The Mre11-Nbs1 interface is essential for viability and tumor suppression http://purl.org/coar/resource_type/c_6501