Perruzza, Lisa Gargari, Giorgio Proietti, Michele Fosso, Bruno D'Erchia, Anna Maria Faliti, Caterina Elisa Rezzonico-Jost, Tanja Scribano, Daniela Mauri, Laura Colombo, Diego Pellegrini, Giovanni Moregola, Annalisa Mooser, Catherine Pesole, Graziano Nicoletti, Mauro Norata, Giuseppe Danilo Geuking, Markus B. McCoy, Kathy D. Guglielmetti, Simone Grassi, Fabio 2017-03-14 The ATP-gated ionotropic P2X7 receptor regulates T follicular helper (Tfh) cell abundance in the Peyer’s patches (PPs) of the small intestine; deletion of P2rx7, encoding for P2X7, in Tfh cells results in enhanced IgA secretion and binding to commensal bacteria. Here, we show that Tfh cell activity is important for generating a diverse bacterial community in the gut and that sensing of microbiota-derived extracellular ATP via P2X7 promotes the generation of a proficient gut ecosystem for metabolic homeostasis. The results of this study indicate that Tfh cells play a role in host-microbiota mutualism beyond protecting the intestinal mucosa by induction of affinity-matured IgA and suggest that extracellular ATP constitutes an inter-kingdom signaling molecule important for selecting a beneficial microbial community for the host via P2X7-mediated regulation of B cell help. application/pdf https://susi.usi.ch/global/documents/318969 https://n2t.net/ark:/12658/srd1318969 https://susi.usi.ch/documents/318969/files/perruzza_celrep_2017.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2017.02.061 info:eu-repo/semantics/altIdentifier/ark/12658/srd1318969 info:eu-repo/semantics/openAccess CC BY-NC-ND Cell reports. - 2017, vol. 18, no. 11, p. 2566-2575 T follicular helper cells IgA P2rx7 ATP Microbiota Metabolism info:eu-repo/classification/udc/61 T follicular helper cells promote a beneficial gut ecosystem for host metabolic homeostasis by sensing microbiota-derived extracellular ATP http://purl.org/coar/resource_type/c_6501