Farsakoglu, Yagmur Palomino-Segura, Miguel Latino, Irene Zanaga, Silvia Chatziandreou, Nikolaos Pizzagalli, Diego Ulisse Rinaldi, Andrea Bolis, Marco Sallusto, Federica Stein, Jens V. Gonzalez, Santiago F. 2019-02-26 The role of natural killer (NK) cells in the immune response against vaccines is not fully understood. Here, we examine the function of infiltrated NK cells in the initiation of the inflammatory response triggered by inactivated influenza virus vaccine in the draining lymph node (LN). We observed that, following vaccination, NK cells are recruited to the interfollicular and medullary areas of the LN and become activated by type I interferons (IFNs) produced by LN macrophages. The activation of NK cells leads to their early production of IFNγ, which in turn regulates the recruitment of IL-6+ CD11b+ dendritic cells. Finally, we demonstrate that the interleukin-6 (IL-6)-mediated inflammation is important for the development of an effective humoral response against influenza virus in the draining LN. application/pdf https://susi.usi.ch/global/documents/318945 https://n2t.net/ark:/12658/srd1318945 https://susi.usi.ch/documents/318945/files/Farsakoglu_CellRep_2019.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2019.01.104 info:eu-repo/semantics/altIdentifier/ark/12658/srd1318945 info:eu-repo/semantics/openAccess CC BY-NC-ND Cell reports. - 2019, vol. 26, no. 9, p. 2307-2315.e5 Natural killer cell influenza vaccine Type I IFN IFNγ IL-6 Dendritic cell info:eu-repo/classification/udc/616 Influenza vaccination induces NK-cell-mediated type-II IFN response that regulates humoral immunity in an IL-6-dependent manner http://purl.org/coar/resource_type/c_6501