<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Chiadò, Alessandro</dc:creator>
  <dc:creator>Varani, Luca</dc:creator>
  <dc:creator>Bosco, Francesco</dc:creator>
  <dc:creator>Marmo, Luca</dc:creator>
  <dc:date>2013-12-10</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">To date, different kinds of biosensing elements have been used effectively for  environmental monitoring. Microbial cells seem to be well-suited for this task: they are  cheap, adaptable to variable field conditions and give a measurable response to a broad  number of chemicals. Among different pollutants, heavy metals are still a major problem  for the environment. A reasonable starting point for the selection of a biorecognition  element to develop a biosensor for metals could be that of a microorganism that exhibits  good mechanisms to cope with metals. Pseudomonads are characterized by the secretion  of siderophores (e.g., pyoverdine), low-molecular weight compounds that chelate Fe3+  during iron starvation. Pyoverdine is easily detected by colorimetric assay, and it is suitable  for simple online measurements. In this work, in order to evaluate pyoverdine as a  biorecognition element for metal detection, the influence of metal ions (Fe3+, Cu2+, Zn2+),  but also of temperature, pH and nutrients, on microbial growth and pyoverdine regulation  has been studied in P. fluorescens. Each of these variables has been shown to influence  the synthesis of siderophore: for instance, the lower the temperature, the higher the  production of pyoverdine. Moreover, the concentration of pyoverdine produced in the  presence of metals has been compared with the maximum allowable concentrations  indicated in international regulations (e.g., 98/83/EC), and a correlation that could be  useful to build a colorimetric biosensor has been observed.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://susi.usi.ch/global/documents/318934</dc:identifier>
  <dc:identifier>https://localhost:5000/ark:/12658/srd1318934</dc:identifier>
  <dc:identifier>https://susi.usi.ch/documents/318934/files/chiado_bios_2013.pdf</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.3390/bios3040385</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/ark/12658/srd1318934</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>CC BY</dc:rights>
  <dc:source>Biosensors. - 2013, vol. 3, no. 4, p. 385-399</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Heavy metals</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Pyoverdine</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Environmental monitoring</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Minimum inhibitory concentration</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Optical detection</dc:subject>
  <dc:subject>info:eu-repo/classification/udc/61</dc:subject>
  <dc:title xmlns:ns6="xml" ns6:lang="en">Opening study on the development of a new biosensor for metal toxicity based on pseudomonas fluorescens pyoverdine</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>