Latorre, Daniela Pulvirenti, Nadia Covino, Daniela Angela Varano, Barbara Purificato, Cristina Rainaldi, Gabriella Gauzzi, Maria Cristina Fantuzzi, Laura Conti, Lucia Donninelli, Gloria Del Cornò, Manuela Sabbatucci, Michela Gessani, Sandra Puddu, Patrizia 2015-12-17 Lactoferrin (LF) exhibits a wide range of immunomodulatory activities including modulation of cytokine and chemokine secretion. In this study, we demonstrate that bovine LF (bLF) up-modulates, in a concentration- and time-dependent manner, CCL1 secretion in monocytes (Mo) at the early stage of differentiation toward dendritic cells (DCs), and in fully differentiated immature Mo-derived DCs (MoDCs). In both cell types, up-modulation of CCL1 secretion is an early event following bLF-mediated enhanced accumulation of CCL1 transcripts. Notably, bLF-mediated up-regulation of CCL1 involves the engagement of distinct surface receptors in MoDCs and their Mo precursors. We show that bLF-mediated engagement of CD36 contributes to CCL1 induction in differentiating Mo. Conversely, toll-like receptor (TLR)2 blocking markedly reduces bLF-induced CCL1 production in MoDCs. These findings add further evidence for cell-specific differential responses elicited by bLF through the engagement of distinct TLRs and surface receptor. Furthermore, the different responses observed at early and late stages of Mo differentiation towards DCs may be relevant in mediating bLF effects in specific body districts, where these cell types may be differently represented in physiopathological conditions. application/pdf https://n2t.net/ark:/12658/srd1318926 https://susi.usi.ch/global/documents/318926 https://susi.usi.ch/documents/318926/files/Latorre_T_2015.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins7124897 info:eu-repo/semantics/altIdentifier/ark/12658/srd1318926 info:eu-repo/semantics/openAccess CC BY Toxins. - 2015, vol. 7, no. 12, p. 5472-5483 Monocyte Dendritic cell Lactoferrin CCL1 Surface receptors info:eu-repo/classification/udc/61 Bovine lactoferrin-induced CCL1 expression involves distinct receptors in monocyte-derived dendritic cells and their monocyte precursors http://purl.org/coar/resource_type/c_6501