Cannavo, Elda Johnson, Dominic Andres, Sara N. Kissling, Vera M. Reinert, Julia K. Garcia, Valerie Erie, Dorothy A. Hess, Daniel Thomä, Nicolas H. Enchev, Radoslav I. Peter, Matthias Williams, R. Scott Neale, Matt J. Cejka, Petr 2018-10-01 DNA end resection plays a critical function in DNA double-strand break repair pathway choice. Resected DNA ends are refractory to end-joining mechanisms and are instead channeled to homology-directed repair. Using biochemical, genetic, and imaging methods, we show that phosphorylation of Saccharomyces cerevisiae Sae2 controls its capacity to promote the Mre11-Rad50-Xrs2 (MRX) nuclease to initiate resection of blocked DNA ends by at least two distinct mechanisms. First, DNA damage and cell cycle-dependent phosphorylation leads to Sae2 tetramerization. Second, and independently, phosphorylation of the conserved C- terminal domain of Sae2 is a prerequisite for its physical interaction with Rad50, which is also crucial to promote the MRX endonuclease. The lack of this interaction explains the phenotype of rad50S mutants defective in the processing of Spo11-bound DNA ends during meiotic recombination. Our results define how phosphorylation controls the initiation of DNA end resection and therefore the choice between the key DNA double-strand break repair mechanisms. application/pdf https://localhost:5000/ark:/12658/srd1318910 https://susi.usi.ch/global/documents/318910 https://susi.usi.ch/documents/318910/files/Cannavo_NC_2018.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-018-06417-5 info:eu-repo/semantics/altIdentifier/ark/12658/srd1318910 info:eu-repo/semantics/openAccess CC BY Nature communications. - 2018, vol. 9, p. 4016 Double-strand DNA breaks Enzyme mechanisms Phosphoproteins info:eu-repo/classification/udc/57 Regulatory control of DNA end resection by Sae2 phosphorylation http://purl.org/coar/resource_type/c_6501