Proietti, Michele Perruzza, Lisa Scribano, Daniela Pellegrini, Giovanni D’Antuono, Rocco Strati, Francesco Raffaelli, Marco Gonzalez, Santiago F. Thelen, Marcus Hardt, Wolf-Dietrich Slack, Emma Nicoletti, Mauro Grassi, Fabio 2019-01-16 T cell dependent secretory IgA (SIgA) generated in the Peyer’s patches (PPs) of the small intestine shapes a broadly diverse microbiota that is crucial for host physiology. The mutualistic co-evolution of host and microbes led to the relative tolerance of host’s immune system towards commensal microorganisms. The ATP-gated ionotropic P2X7 receptor limits T follicular helper (Tfh) cells expansion and germinal center (GC) reaction in the PPs. Here we show that transient depletion of intestinal ATP can dramatically improve high-affinity IgA response against both live and inactivated oral vaccines. Ectopic expression of Shigella flexneri periplasmic ATP-diphosphohydrolase (apyrase) abolishes ATP release by bacteria and improves the specific IgA response against live oral vaccines. Antibody responses primed in the absence of intestinal extracellular ATP (eATP) also provide superior protection from enteropathogenic infection. Thus, modulation of eATP in the small intestine can affect highaffinity IgA response against gut colonizing bacteria. application/pdf https://n2t.net/ark:/12658/srd1318837 https://susi.usi.ch/global/documents/318837 https://susi.usi.ch/documents/318837/files/Proietti_NatCommun_2019.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-018-08156-z info:eu-repo/semantics/altIdentifier/ark/12658/srd1318837 info:eu-repo/semantics/openAccess CC BY Nature Communications. - 2019, vol. 10, p. Art. number: 250 Gastroenterology Mucosal immunology info:eu-repo/classification/udc/61 ATP released by intestinal bacteria limits the generation of protective IgA against enteropathogens http://purl.org/coar/resource_type/c_6501